Delivery Of The Cas Components - 1306 Words

Delivery of CRISPR-Cas Components: CRISPR systems are generally delivered in the cell using normal methods of transfection such as microinjection, gene gun, electroporation, sonoporation, and using viruses such as adenoviruses and lentiviruses. In cultured mammalian cells, researchers have used electroporation, nucleofection, and Lipofectamine mediated transfection methods to deliver vectors expressing the gene for gRNAs and Cas9 endonuclease. In cultured human and mouse cells, Lentiviral vectors have also been used as a mode of delivery of genes. In addition to animal models and cell lines, Cas9 has been used successfully in a lot of plant species including wheat, rice, sorghum, tobacco, and thalecress using a range of standard delivery methods such as PEG-mediated transformation of protoplasts, Agrobacterium-mediated transfer in embryos and leaf tissue, and/or bombardment of callus cells with plasmid DNA. [1] As a novel method of gene delivery, scientists at North Carolina State University and University of North Carolina at Chapel Hill have made use of a nanoscale vehicle made of DNA to deliver CRISPR Cas9 complex into both cell cultures as well as animal models. This method relies on directly delivering the Cas9 protein in the cell instead of transfecting DNA into the cell. This method of delivery has been commonly stated as the Nanoclew mechanism of delivery. This method is highly compatible since the delivery system is made of DNA. The DNA is engineered to partiallyShow MoreRelatedExploring Data Sources And Gathering Appropriate Information783 Words   |  4 Pagesthe completion year, the project size (gross area), budgets and actual costs, building types, delivery methods, green features, number of RFIs, change orders, number of project participants, and other components. For instance, 30 case studies with full-implemented LIB are represented in Table 2. 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